基于相对熵排序的建筑工程项目投标决策

投标决策是施工企业面对的重要问题．在建筑工程项目投标问题上，先建立风险指标体系，再结合层次分析法和信息熵法求得风险指标的综合权重，然后借助相对熵排序法，对各个方案进行综合评价，得到有效反映各方案差异的评价模型，通过对比可知此法可行有效，且灵敏度高．     

血浆D-二聚体和超敏C反应蛋白检测在急性脑梗死中的临床价值

目的探讨血浆D-二聚体(D-dimer,D-D)及超敏C反应蛋白(high-sensitivity C-reactive protein,hs-CRP)水平与急性脑梗死(acute cerebral infarct,ACI)的关系。方法采用免疫透射比浊法检测102例ACI患者和50例正常对照者血浆D-D和hs-CRP水平。根据梗死灶大小102例ACI患者分为大梗死灶组、中梗死灶组和小梗死灶组,并进行相关统计分析。结果 ACI组患者血浆D-D和hs-CRP水平显著高于正常对照组(P0.01);大梗死灶组D-D和hs-CRP水平明显高于中、小梗死灶,而中梗死灶组D-D和hs-CRP水平高于小梗死灶组。结论监测血浆D-D和hs-CRP能为ACI的临床诊断、病情判断提供重要的参考依据。     

基于GIS的秦巴山区土地生态敏感性评价──以陇南山区为例

生态环境敏感性评价是进行生态功能区划的前提条件,也是判断生态系统稳定性的重要手段。进行土地生态环境敏感性评价,是落实科学发展观和生态文明理念,加快实施主体功能区规划和建设国家生态安全屏障的重要举措,对推动陇南市可持续发展和维护国家生态安全具有重要意义。笔者根据陇南市山地生态系统基本特征,运用GIS技术,从土壤侵蚀、石漠化、和地质灾害3个方面对陇南市土地生态敏感性展开单因子评价和综合评价,并对综合评价结果进行趋势面分析。评价结果表明:(1)陇南市土地生态敏感性区域差异比较明显,在东西方向上为东高西低,整体呈递减趋势,在南北方向上呈现较为明显的"n"字型,南部略高于北部;(2)运用自然断点法,将陇南市土地生态敏感性分为极敏感区、高敏感区、中敏感区、低敏感区和不敏感区,分别占市域总面积的19.18%,24.61%,25.71%,16.88%和13.61%;其中极敏感区和高敏感区主要分布在宕昌县和武都区等周边地区,该区生态敏感极强,需要加强保护,开发强度不宜过高;低敏感和不敏感区域主要分布在礼县、西和县、徽县和成县等大多数区域,可以适当进行开发。     

4 株致病性大肠杆菌致犊牛腹泻的分离鉴定及毒力与耐药性分析

[目的]为查明致北疆地区某牧场犊牛腹泻的病原,对脱水濒死10日龄新生犊牛进行剖检,无菌采集充血肠系膜淋巴结2 份。[方法]通过采用培养、染色镜检、生化特性考察、PCR鉴定等方法来分离鉴定致病菌,并对分离致病菌株进行相关毒力基因检测及药物敏感性试验。[结果]经分离鉴定分离出4 株大肠杆菌,毒力基因PCR检测结果K99、Sta、irp2、Eae基因分别在163 bp、314 bp、301 bp、552 bp处有清晰条带,与预期[目的]片段大小相符;药物敏感性试验结果表明,分离株对头孢哌酮、链霉素、庆大霉素敏感,对恩诺沙星、卡那霉素高度敏感。[结论]犊牛腹泻要以预防为主,治疗建议采用对因治疗,并配合提高抵抗力的中兽药进行。     

1株奶牛源约翰逊不动杆菌的分离鉴定

旨在了解从奶牛垫料中分离得到1株革兰阴性菌的病原特征,本研究通过形态学观察、生化试验、染色镜检、16S rDNA同源性分析等方法对分离菌进行鉴定,并采用药敏试验测定药物敏感性,小鼠攻毒试验确定分离菌株毒力情况。结果表明,分离菌为革兰阴性菌;扩增的16S rDNA序列长度为1 283 bp,与约翰逊不动杆菌的核苷酸相似性大于99%;分离菌株对小鼠有较强的致病性,试验组小鼠死亡率达80%。分离菌对链霉素、复方新诺明等6种药物敏感,对头孢噻肟、头孢呋辛中度敏感,对青霉素、红霉素等4种药物耐药。本研究从奶牛垫料中分离到1株约翰逊不动杆菌,为奶牛源约翰逊不动杆菌的防治和研究提供了参考依据。     

豪猪源肠侵袭型大肠杆菌的分离鉴定及致病性研究

【目的】 查明引起福建省龙岩市某豪猪养殖场致豪猪腹泻死亡的病原菌及其特征,为该病的科学防控及合理用药提供参考依据。【方法】 分离腹泻死亡豪猪的病原,并结合形态特征、生理生化试验、16S rRNA基因扩增、种系发育分群鉴定分离菌株;通过毒力基因检测、动物致病性试验及药敏试验对分离菌株的致病性和耐药性进行研究。【结果】 从发病死亡豪猪肝脏组织中分离到1株大肠杆菌,命名为Fj/Porcupine2018,该分离菌株与22株不同来源的参考菌株16S rRNA序列之间的相似度为97.4%~99.8%,其中与禽源分离株（登录号：MN022583）和人源分离株（登录号：MW881377）的序列相似度高达99.8%;种系发育分群证实该分离菌株属于B1群。毒力基因检测结果显示,该分离菌株除检出肠侵袭型大肠杆菌毒力基因EinV外,还检出papC、iroC、Afa、luxs、stx2f及ompA 6种毒力相关基因。动物致病性试验显示,该分离菌株对小鼠具有较强的致病性,小鼠在攻毒后31 h内全部死亡,且肝脏、脾脏、肺脏及肠道等器官组织均有明显的病理损伤;在对常见抗菌药物的药敏试验中,该分离菌株对大环内酯类、四环素类、喹诺酮类、磺胺类、头孢类、氨基糖苷类、青霉素类7类11种药物表现出不同程度的耐药,耐药率为25%~100%,仅对头孢曲松和头孢西叮2种药物表现敏感。【结论】 本研究分离获得了1株腹泻死亡豪猪的病原菌大肠杆菌,通过一系列生物学特性研究证实该分离菌为1株具有较强致病力且呈现多重耐药性的B1群肠侵袭型大肠杆菌,为豪猪等野生动物大肠杆菌病的防控提供了重要的参考依据。     

小麦品种对绿麦隆反应敏感性的遗传研究

本研究以对绿麦隆反应敏感的苏麦3号、望水白及宁7840和不敏感的宁麦6号及扬麦5号五个小麦品种为亲本,在冷温室内、在有效用药量为0.15克/米~2的条件下,观察了绿麦隆对亲本、F_1及部分 F_2和回交后代苗期表现的影响。结果表明,对绿麦隆反应的敏感性的差异受一对基因控制,敏感为隐性,不敏感为显性。建议把苏麦3号和望水白中的     

Genetic control of photoperiodic sensivity and maturity in spring wheat within narrow limits of adaptation

Summary Photoperiodic respose, as assessed by a regression technique, exhibited complete dominance averaged over the crosses of an eight parent diallel in the vernalized condition. Photoperiodic response as final leaf number for the vernalized 8-hour photoperiod diallel was closely related to photoeriodic response of the regression method. However, the diallel analyses of both sets of data showed little agreement in terms of respectieve array positions.The inheritance of photoperiodic response in diallels using regression values showed little agreement between the vernalized and unvernalized conditions. This difference was postulated to be due to interaction of vernalization and photoperiodic response in the unvernalized situation. In the unvernalized condition photoperiodic response exhibited non-allelic interaction, attributable mainly to the cultivar Pinnacle in general behaviour in its crosses. Its removal gave a situation of high average dominance for photoperiodic response with a clear indication that high photoperiodic sensitivity was dominant to comparative insensitivity.Days to ear emergence (vernalized and 18-hour photoperiod) exhibited non-allelic interaction in its expression, due mainly to the general behaviour of the cultivar Pinnacle in its crosses. Removal of its array gave a situation of a moderately strong degree of overdominance in the expression of days to ear emergence. Maturity differences amongst parents and F₁'s, vernalized and under 18-hour photoperiod, are postulated to be due to a factor other than vernalization or photoperiodic response beheved to be growth temperature in differentially in fluencing growth and/or developmental rates between genotypes.     

Effects of loci determining photoperiod sensitivity (Ppd-H1) and vernalization response (Sh2) on agronomic traits in the 'Dicktoo'×'Morex' barley mapping population

The objectives of this research were to determine the individual and interaction effects of the Ppd-H1 and Sh2 loci on agronomic traits under short- and long-photoperiod regimes. Nineteen doubled haploid (DH) lines from the ‘Dicktoo’בMorex’ mapping population, which represented the four genotypes at the Ppd-H1 and Sh2 loci, were pheno-typed in controlled environment photoperiods. Both Ppd-H1 and Sh2 had significant effects on several agronomic traits, in addition to their role in determining first node appearance and flowering time. The magnitude of these effects depended on daylight. Under long-day conditions (18 h) Ppd-H1, and under short-day conditions (12 h) Sh2 was a significant determinant of most characters. The interactions between these two loci were significant for several characters, particularly for yield components, under both long- and short-photoperiod regimes. Under the long-day treatment, Ppd-H1 influenced plant height through the determination of node number. There was an epistatic association between the two loci for both 1000-kernel weight and tillering. The combination of photoperiod insensitivity and vernalization requirement caused a significant increase in tillering. This was paralleled by a decrease in 1000-kernel weight. Under the long-day treatment, neither Ppd-H1 nor Sh2 influenced plant yield. Under short-day conditions, the combination of photoperiod insensitivity and vernalization requirement had a pronounced negative effect on plant yield.     

Comparison of Insulin Sensitivity of Horses Adapted to Different Exercise Intensities

Diets high in concentrates and soluble carbohydrates are associated with reduced insulin sensitivity in horses. Exercise training could protect against diet-induced insulin resistance. The objective of this study was to determine the intensity of exercise training required to affect insulin sensitivity in stabled horses fed a diet high in concentrates but moderate in soluble carbohydrates. In all, 31 stabled horses underwent three different exercise regimens: turnout, light exercise, and moderate exercise, while being fed a diet containing 60% concentrate. Blood was sampled monthly and analyzed for insulin. Insulin sensitivity was assessed using basal insulin concentrations and calculated insulin sensitivity (reciprocal of the square root of insulin) and compared across months by analysis of variance with repeated measures. Insulin sensitivity (reciprocal of the square root of insulin) was higher during periods of moderate and light physical activity as compared with turnout. These results indicate that turnout alone may not be adequate to improve insulin sensitivity in horses fed high amounts of concentrate.